Similarly to plotGeneralEnrichment and plotCommunitiesEnrichment, the results of the TF-based enrichment analysis are plotted. This function produces multiple plots. First, one plot per community to summarize the TF-specific enrichment. Second, a summary heatmap of all significantly enriched terms across all TFs and for the whole eGRN. The latter allows to compare the results with the general network enrichment. Third, a subset of the aforementioned heatmap, showing only the top most significantly enriched terms per TF and for the whole eGRN (as specified by nID) for improved visibility .

  rankType = "degree",
  n = NULL,
  TF.IDs = NULL,
  topn_pvalue = 30,
  p = 0.05,
  nSignificant = 2,
  nID = 10,
  display_pAdj = FALSE,
  maxWidth_nchar_plot = 50,
  outputFolder = NULL,
  basenameOutput = NULL,
  plotAsPDF = TRUE,
  pdf_width = 12,
  pdf_height = 12,
  pages = NULL,
  forceRerun = FALSE



Object of class GRN


Character. One of: "degree", "EV", "custom". This parameter will determine the criterion to be used to identify the "top" nodes. If set to "degree", the function will select top nodes based on the number of connections they have, i.e. based on their degree-centrality. If set to "EV" it will select the top nodes based on their eigenvector-centrality score in the network.


NULL or numeric. Default NULL. If set to NULL, all previously calculated TF enrichments will be plotted. If set to a value between (0,1), it is treated as a percentage of top nodes. If the value is passed as an integer it will be treated as the number of top nodes. This parameter is not relevant if rankType = "custom".


NULL or character vector. Default NULL. For rankType="custom" the IDs of the TFs to plot. Ignored otherwise.


Numeric. Default 30. Maximum number of ontology terms that meet the p-value significance threshold to display in the enrichment dot plot


Numeric. Default 0.05. p-value threshold to determine significance.


Numeric > 0. Default 3. Threshold to filter out an ontology term with less than nSignificant overlapping genes.


Numeric > 0. Default 10. For the reduced summary heatmap, number of top terms to select per community / for the general enrichment.


TRUE or FALSE. Default FALSE. Is the p-value being displayed in the plots the adjusted p-value? This parameter is relevant for KEGG, Disease Ontology, and Reactome enrichments, and does not affect GO enrichments.


Integer (>=10). Default 50. Maximum number of characters for a term before it is truncated.


Character or NULL. Default NULL. If set to NULL, the default output folder as specified when initiating the object in initializeGRN will be used. Otherwise, all output from this function will be put into the specified folder. If a folder is provided, while we recommend specifying an absolute path, a relative one also works.


NULL or character. Default NULL. Basename of the output files that are produced. If set to NULL, a default basename is chosen. If a custom basename is specified, all output files will have the chosen basename as file prefix, be careful with not overwriting already existing files (if forceRerun is set to TRUE)


TRUE or FALSE. Default TRUE.Should the plots be printed to a PDF file? If set to TRUE, a PDF file is generated, the name of which depends on the value of basenameOutput. If set to FALSE, all plots are printed to the currently active device. Note that most functions print more than one plot, which means you may only see the last plot depending on your active graphics device.


Number>0. Default 12. Width of the PDF, in cm.


Number >0. Default 12. Height of the PDF, in cm.


Integer vector or NULL. Default NULL. Page number(s) to plot. Can be used to plot only specific pages to a PDF or the currently active graphics device.


TRUE or FALSE. Default FALSE. Force execution, even if the GRN object already contains the result. Overwrites the old results.


The same GRN object, without modifications.


# See the Workflow vignette on the GRaNIE website for examples
GRN = loadExampleObject()
#> Downloading GRaNIE example object from
#> Finished successfully. You may explore the example object. Start by typing the object name to the console to see a summaty. Happy GRaNIE'ing!
GRN = plotTFEnrichment(GRN, n = 5, plotAsPDF = FALSE, pages = 1)
#> Error in .checkGraphExistance(GRN): could not find function ".checkGraphExistance"